|Voice actor(s)||Kathy Levin|
|Game(s)||Pajama Sam: "No Need to Hide When It's Dark Outside"|
Pajama Sam 2: Thunder and Lightning Aren't So Frightening (cameo)
The Trees are three 'high-class' tree guards that only let other trees pass through their area. Pajama Sam therefore has to "disguise" himself as a tree to go forward. It leads to Darkness' garden. and they are neighbors from Carrot. Their names are Sebastian, Valeria, and Algernon.
The Tree Guards are shown to be old with visible wrinkles on their faces.
The Tree Guards block off the path to Darkness' garden. Though Sam can speak to them, he can't get past them as he is not a tree. Sam will need to use a log to place on his head so he can get past the trees without a problem. The same goes for when Sam has to leave the garden.
The trees appear in all versions of the game, but it is only important to get past them in the Salad Liberation storyline or if the Brain Tickler question about the "Land of Darkness" asks about what color the flowers are.